FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3603555
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG345570.9589696841036144No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN200960.5576715215946475No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC131390.3646121677066119No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC127070.35262400601628113No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG113320.31446724137691806No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG111030.3081124056660714No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG89900.24947586480572656No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA77660.21550940668312266No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73920.20513076670121586No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT66360.184151483743137No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG58870.1633664534050403No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG58080.16117417383666963No Hit
GTGGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGG57910.1607024174738557No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC56020.15545759673433596No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT54770.1519887999489393No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG50590.14038914349857293No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA50260.1394733811472282No Hit
GCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGC48320.13408980853629263No Hit
GGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGC48080.13342379955349648No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT43870.12174089198028058No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG43390.12040887401468826No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC43090.11957636278619307No Hit
TGGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGG42410.11768933733493732No Hit
GCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGC41330.11469229691235461No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC39990.1109737467584094No Hit
TGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA39520.10966947916710026No Hit
GCCGGGAGGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGG38900.10794895596154352No Hit
GGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCG38630.10719969585589785No Hit
GGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAG38440.10667243874451755No Hit
ACTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG38180.10595092901315506No Hit
CGCTTCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA37060.10284288709343967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG11950.047.65021570
CGCGGCA100450.037.98586732
GCGGCAC100700.037.77739733
CGGCACC101500.037.30420334
ACGATCG754.3388354E-836.487266
TAGTCCG5600.036.039316
CCGTCGC102000.035.62183845
CCCGTCG102800.035.4505644
GCACCCC107200.035.4340836
GGCACCC107450.035.26045635
TCGCCGG103500.035.2371348
CACCCCC110550.034.42884437
CCCCCGA111900.034.0631619
TCAGGAT23800.033.3317170
GCAGTCC16750.032.8793755
CCCCCGT110250.032.80923542
ACCCGCG119150.032.1080725
CGCTTCC8650.031.2806111
GGCAGTC16550.031.0079044
CCCCGAC123750.030.80164720
Produced by FastQC (version 0.11.5)