FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3555096
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN194890.5481989797181286No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC111470.3135499013247462No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA104070.29273471096139175No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC93080.2618213404082478No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG75860.21338382986000942No Hit
GGGCGGCGGCGGGGGCACGGTCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGCCGGCGGCGGCGGCGACTCTGG70430.19810998071500743No Hit
GGGATTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGA54290.15271036281439376No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC52400.14739405068105052No Hit
GGCCGGCGGCGGGGGCACGGTCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGCCGGCGGCGGCGGCGACTCTGG43570.12255646542315594No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT43450.1222189217956421No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG43080.12117816227747435No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT41210.11591810741538343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG16500.058.6456835
TACGGCC16650.057.706627
GTCTACG16850.057.4242744
CTACGGC17400.055.4157756
GCGCGGT54000.051.921539
CCGAACT7700.049.43945767
GCCATAC19600.049.19494211
CGCGGTG57550.048.65931310
CATACCA19900.048.45125213
ACGGCCA20050.048.2620358
TGGCGCG58100.048.08030315
GCCGGTT8000.047.439713
GACTCGC7150.046.3846933
CGGCCAT20850.046.2456059
GGCCATA21250.045.375110
CGGTTCA8300.044.903685
CGGATTT5700.044.56061
CCGGTTC8550.044.3880274
GGGTCTA15950.044.3729862
CCATACC21800.044.07159812
Produced by FastQC (version 0.11.5)