FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3704560
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG121960.32921588528732154No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC77890.21025438918522038No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC64610.17440667717623684No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG53230.14368777938540608No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG39840.10754313602695056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA37800.037.43231632
GCGGCAC40050.035.42268433
TCGCCGG39000.034.65407648
CGGCACC42450.033.1824934
CCCGTCG41350.032.72303444
CCGTCGC42100.032.3145445
CCCCCGA48200.030.08762619
GCACCCC48900.029.31262836
CACCCCC49900.028.73454337
CGTACGT2050.028.70225119
ACCCGCG49750.028.40873725
CCCCGAC52050.027.8655120
CAGTCCG50700.027.4356866
AGTCCGC52600.027.2340567
GGCACCC52400.027.01733235
GTTCCGT3350.026.9570061
CCCCCGT50200.026.9380442
TTATCGG2250.026.1435247
GTCCGCC56100.025.7816758
TCCGCCC57000.025.6781229
Produced by FastQC (version 0.11.5)