FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3704560
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA61550.16614658690910664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA2750.042.980491
GCGCGGT20150.042.2334259
CGGGTAG5900.041.2449871
TGGCGCG22000.038.83450315
CGGCAAT4050.035.1928141
GGGGACT36250.034.9074821
CGGCATT6050.034.476331
CGGTATG2550.034.0819931
CTACTCG25550.033.82642737
CGGCAAA8550.033.747151
TACTCGG25650.033.42980238
GGGGGGA77500.033.014241
GGGGTAT16150.032.9339681
CGGGTAT4650.032.894621
CGGCACA8200.032.219951
CGGTATC1950.032.0895081
CGCGGTG26900.032.0216210
GGGGTAC13800.031.9926191
GGGGTAG18150.031.9863721
AGGATCG26200.031.40750959
Produced by FastQC (version 0.11.5)