FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3680907
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA61130.16607319880670715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT18250.041.145699
TGGCGCG18800.039.9419615
GGGGTAG19150.038.9168431
CGGGTAG6150.036.2407761
TACTCGG21450.035.6128638
CTACTCG21950.034.9469637
GGGGGGA74900.034.127691
AGGATCG22450.033.8343159
CGGCATA2700.033.5353051
CGGTAAG2750.032.925571
GATCGCT23750.032.16641261
GCCGGTT4650.032.001663
CGCGGTG23900.031.70835510
GGGGTAT14650.031.6159821
AGTACGC6900.031.55758764
ACTCGGG24500.031.3305539
CGGATTG4700.031.1203211
CGGCAAC7250.030.7421761
CGGCAAT4200.030.6792741
GGGGACT35500.030.6068691
Produced by FastQC (version 0.11.5)