FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3687010
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG123870.3359632873249598No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC76620.20781066501040138No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC64540.1750469892948487No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG52350.14198496885009806No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG40570.11003496057781237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA36000.037.66138532
GCGGCAC38850.034.9028533
CCGTCGC39450.033.51983645
TCGCCGG39250.033.46345548
CGGCACC40750.033.1962434
CCCGTCG40650.032.43719544
CCCCCGA46450.029.67162319
GCACCCC46850.029.40664136
ACCCGCG47650.029.01241725
CACCCCC49050.028.45072437
CCCCGAC50350.027.64918720
GTTATCG2650.027.4335386
TTATCGG2300.027.092757
GATAGGC25800.027.07198740
GGCACCC51600.026.6287635
CGCCCTA10750.026.42926861
AGTCCGC53600.026.3514067
CCCCCGT50350.026.24147642
ATGCCCG11600.026.2164170
CAGTCCG52000.025.8307386