FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3687010
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA59780.16213679919501167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT18200.042.5869839
CGGCATA3050.038.8318631
TGGCGCG21000.037.40252715
CGGGTAG5500.036.7345281
CGGGTAT4400.036.417851
CGGTAAG2700.036.1246341
GGGGTAG17550.034.9337121
AGTACGC4900.034.40601364
CGGTATT2950.034.2440531
GGGGGGA77350.033.8211821
CGGCACA7650.033.6960871
CGGTATA2300.033.3199231
GGGGTAT16300.033.1247981
CGGCAAC6650.033.0010721
CGGCAAA8150.032.911091
CGCGGTG24100.032.7354310
CTACTCG24500.032.0101537
AGGATCG24350.031.78151159
CGGATAA3900.031.2617021
GGGGTGT28500.031.167681
Produced by FastQC (version 0.11.5)