FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3686406
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA57760.1566837727586164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT18450.042.938739
CGGCATA3250.041.8604661
TGGCGCG21100.038.03368415
GGGGTAG17300.036.4968451
CGGTAAG3250.036.493741
CGGGTAG5600.036.129571
CTACTCG22550.035.70490637
TACTCGG22800.035.1693438
CGGCAAT4500.034.108531
CGGCAAA8750.033.8870471
CGGGAAT7700.033.5246161
GGGGACT36400.033.062871
GTCGTAC958.58563E-932.7695123
CGGTATA2450.032.7479861
AGGATCG24000.032.4027859
GGGGTAT15900.032.0315971
GGGGGGA78300.031.9433331
CGGATAA3650.031.5387081
CGCGGTG25350.031.52419910
CGGCATT6000.031.3953511
Produced by FastQC (version 0.11.5)