FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3742164
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG235520.6293684616708407No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA221440.5917431732013883No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC179490.4796422604674728No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC159860.42718598115956435No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN95720.25578782757784No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT84380.22548450575656226No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG66560.1778650000374115No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA47320.12645089846409724No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG45520.12164084738135474No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA42440.11341031552866201No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC41100.10982949972262038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG46950.063.7110825
GTCTACG46950.063.638024
TACGGCC48350.062.0766457
CTACGGC48350.061.9372446
TCGCGTA352.7051647E-760.8391260
CGGCCAT52300.057.254789
ACGGCCA52800.057.1023758
GCGTCTA9900.056.497112
CGTCTAC11500.055.4816323
GGGTCTA38400.055.2244072
GCCATAC54300.054.89327211
GCGCGGT89100.053.9336289
CCATACC55750.053.5878912
GGCCATA56600.053.02623710
CATACCA56300.052.88161513
CGCGGTG92900.051.7644410
GCCGGTT13750.051.3923723
CCTGGTT83550.050.66913270
CGGTTCA13950.050.655565
TGGCGCG94450.050.62373715
Produced by FastQC (version 0.11.5)