FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3745240
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG234190.6253003812839765No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA214110.5716856596640002No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC178840.47751278956755777No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC160530.4286240668154778No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN94230.25159936345868356No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT86250.23029231771528658No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG66930.1787068385470624No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA47820.1276820711089276No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG45220.1207399258792494No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA42240.11278315942369514No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC40970.1093921884845831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG46200.063.600294
TCTACGG46750.062.9253855
TACGGCC47400.062.1339877
CTACGGC48100.061.2993936
ACGGCCA51200.057.85738
CGGCCAT51550.057.264959
GGGTCTA39350.056.1998522
GCCATAC52450.056.1500911
GCGCGGT89300.055.4408849
CCATACC53350.055.07432612
CATACCA53350.054.8815313
CCGAACT14950.054.64016367
GCCGGTT15100.053.8128283
GGCCATA55500.053.2511110
GCGTCTA8900.052.777742
CGCGGTG94700.052.4605510
CGTCTAC10850.051.8237153
CGGTTCA15650.051.484175
CCTGGTT82450.051.2520270
TGGCGCG97500.050.9512615