FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4761511
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG300740.6316062275189536No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN232930.48919345140649684No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG116070.24376715710622113No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG78940.16578770898565603No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC68670.14421892546294654No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA67200.141131670177807No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG54610.1146904837561018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA91150.037.6433332
GCGGCAC95200.036.20938533
CGGCACC97050.035.5798934
CCCGTCG93300.034.6678744
TCGCCGG93700.034.3601748
CCGTCGC94050.034.12328345
GCACCCC103150.033.5165836
CCCCCGA104700.032.96473319
ACCCGCG106050.032.4547725
CACCCCC107100.032.21040337
GGCACCC107700.031.95427335
GTTCCGT4500.031.3271371
CCCCGAC111550.030.9591120
TAGTCCG6300.030.471136
CCCCCGT109550.029.1567442
AGGAGGG158800.028.71659970
CCCGACC124050.027.9633121
CGTCGCC118700.027.11635846
CCGCGCG127400.027.0255727
AGTCCGC128800.026.907697