FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4761511
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN229220.48140180711543035No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC164770.346045614511864No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC132730.27875605033780243No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC79900.16780387570248184No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT77430.1626164467539821No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA54160.1137454056075897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT86650.051.887359
CGCGGTG92000.048.94603310
TGGCGCG92250.048.9263815
CCGAACT14150.047.85539267
GCCGGTT14250.046.4138683
CGGTTCA15300.043.8996285
CGCGTGC103850.043.8757318
GGCGCGT105150.043.37937516
GCGTGCC105950.043.16480619
CGTGCCT106350.042.90008520
CTACTCG105950.042.4655237
TACTCGG105600.042.17408838
GTAGTCC107000.042.09878527
TAGTCCC107850.041.7700628
TGTAGTC109150.041.40115426
GCGCGTG111350.041.01685317
CGGCAAT4150.040.614721
GCCGAAC16350.040.5787566
CCTGTAG112000.040.41583324
ACTCGGG112550.040.0288539