FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4785218
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN230460.48160815243945No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC172960.36144643775894847No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC138540.2895165904667248No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC85730.17915589216625033No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT81080.16943846654426192No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA56630.11834361569316174No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG50250.10501088978600347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT88800.050.464099
CCGAACT13600.050.02322867
CGCGGTG95550.046.93057310
TGGCGCG95050.046.60377515
GCCGGTT15050.045.1074753
CGGTTCA15350.044.449265
TACTCGG108500.041.75906438
CTACTCG107900.041.69927637
CGTGCCT107150.041.65683420
GGCGCGT108350.041.2945116
GCCGAAC16750.041.0877966
GTAGTCC109600.040.97879427
TAGTCCC110100.040.94396628
GCGTGCC109100.040.8558519
CGCGTGC110550.040.44891718
CCGGTTC17100.040.1008644
TGTAGTC113450.039.85894426
ACTCGGG115950.039.4923239
CGAACTT17800.039.07755368
TACGGCC6500.039.033567