FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4929592
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG334080.6777031446010137No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN243500.49395568639351894No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG126290.25618753032705344No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG87970.17845290239029923No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC73000.14808527764569562No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA71440.1449207155480616No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG60800.12333677918983964No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC51550.10457254880322754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA96600.036.51943232
GCGGCAC100850.035.10799433
CGGCACC101000.034.97168734
TCGCCGG98300.034.18254548
CCGTCGC98450.033.89523745
CCCGTCG98750.033.8709744
GCACCCC107100.033.1567936
ACCCGCG110500.032.41339525
CCCCCGA109550.032.21286819
GGCACCC112900.031.46112335
CACCCCC115100.031.0254437
CCCCGAC117050.030.31015820
GTTCCGT5850.029.9411351
CCCCCGT116150.028.78660242
TATCGCG605.6180486E-428.57847210
AGGAGGG172050.027.56414270
CCCGACC129100.027.46452921
CCCGCGC130850.027.38925726
AGGACCG8300.027.20832370
GTGCCGT9650.027.0483151