FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4929592
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN239870.48659199382017826No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC175930.3568855191261265No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC140720.2854597297301683No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC87780.17806747495533098No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT81800.16593665358106716No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA60140.12199792599468678No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG52970.10745311173825339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT87650.050.3104489
CCGAACT14750.049.0884567
CGGTTCA15600.047.4708985
CGCGGTG93750.047.1100710
TGGCGCG93850.046.942615
GCCGGTT16450.044.1847763
GCCGAAC17150.043.05924266
CCGGTTC17800.041.41114
CGCGTGC107850.041.3534818
CTACTCG108650.041.20332337
GTAGTCC109300.041.20031727
CGTGCCT108450.041.10334420
GGCGCGT108800.041.06035216
TACTCGG109300.041.04812638
GCGTGCC109350.040.65122619
TAGTCCC111300.040.48489828
TGTAGTC111750.040.48287226
CTTAGGC17650.040.46147518
CGAACTT18750.040.055168
ACTCGGG116800.038.94753339