FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3780254
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG173220.4582231775960028No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC112640.29796939570727254No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC109260.28902819757614173No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG76740.2030022321251429No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG66330.1754644000112162No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT51580.13644585787092614No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT50550.13372117323333302No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG47780.12639362328563106No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC46640.12337795291004255No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG45420.12015065654318467No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT44620.11803439663049098No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG44120.1167117341850574No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40730.10774408280501786No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG39350.10409353445562124No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA38430.10165983555602348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT27500.048.69693870
AGGACCG9950.035.82230870
GTTCCGT4900.035.2097241
CGCGGCA51650.034.59729832
ATGGCCG30900.034.59494869
GCGGCAC53000.033.9873133
TAGTCCG3900.033.5156946
CGGCACC54050.033.020434
CCGTCGC52250.032.5709945
CCCGTCG52800.032.53817444
TCGCCGG53650.031.5920548
ACGATCG802.8285795E-630.0979756
GCACCCC60300.029.85237336
CACCCCC61350.029.30103937
GGCACCC61650.029.1316535
CCCCCGA62350.028.8696719
GATAGGC34850.028.27138540
TCAGGAT22400.027.95990670
CGTACTA1750.027.5177842
TTCCGTT5900.027.4011842