FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3879377
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG312430.8053612732147455No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC129860.3347444705683412No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA118340.3050489808028454No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC111200.2866439637086058No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG84150.21691627289639548No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT70690.18221998016691854No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG58880.15177694769031214No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC53900.138939834927103No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41470.1068986076888119No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC40630.10473331156007781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG60500.064.371624
TCTACGG61100.063.570575
TACGGCC61950.062.4343577
CTACGGC62750.061.6844646
ACGGCCA64000.060.4882748
CCTGGTT94050.058.94610670
GGGTCTA47550.058.7531662
CGGCCAT66800.057.7982949
GCCATAC67100.057.33474711
GCGTCTA10850.057.0779722
CCATACC69450.055.64243712
CATACCA69950.055.24185613
GGCCATA70700.054.75600410
CGGTCTA8400.053.6559372
CGTCTAC14250.052.148553
GCCGGTT10700.052.087633
TACCACC73850.052.0821715
GCGCGGT63350.051.9833079
CTGAACG75350.051.33922622
TGAACGC75950.051.0822523