FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3829888
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG303240.7917725009190869No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC132250.34531035894522244No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA116750.30483920156411887No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC109840.2867968984993817No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG84690.22112918184552655No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT66730.1742348601316801No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG55860.1458528291166739No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC51770.13517366565288594No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40470.1056688864008556No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC38870.10149121854216103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG59350.064.521764
TCTACGG60150.063.6097455
TACGGCC61000.062.4977577
CTACGGC61900.061.589076
ACGGCCA63600.060.0510188
GGGTCTA45450.059.8032762
CGGCCAT64750.058.771159
CGTCTAC13650.057.9731563
CCTGGTT94750.057.42123870
GCCATAC66600.056.9319711
GCGTCTA12150.056.918252
CCATACC69050.054.85995512
GGCCATA70350.054.19066210
CATACCA70300.054.0809613
CGGTCTA7650.053.520152
CCGAACT11900.053.1526167
TACCACC72400.052.4647915
GCGCGGT63200.051.991839
GCCGGTT12150.050.4052163
TGAACGC75350.050.3945223