FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3660329
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN464671.2694760498304933No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG227540.6216381095797673No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG116860.31926091889554187No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC96900.26473030156578825No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG93050.2542121213694179No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC88890.24284702276762554No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT63590.1737275529057634No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG62300.1702032795412653No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA60630.16564084813141114No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT52670.14389416907605845No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA52100.1423369320080244No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC45780.12507072451683987No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC44970.12285780868331782No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT42820.11698401974248762No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT42580.11632834097699961No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG42330.11564534226294959No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC42110.11504430339458556No Hit
GGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAG39300.10736739784866334No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG39210.10712151831160532No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG39040.10665707918605132No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG38370.10482664263239726No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG38030.10389776438128923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3250.048.29189340
GCGGCAC71250.037.2325233
CGCGGCA71050.037.18054632
CGGCACC73150.036.18050434
CGTATGC4800.035.6737741
TCGCCGG70700.035.6608848
CCGTCGC72450.034.5416145
CCCGTCG72400.034.4949744
GTGCCGT6850.034.4435961
GCACCCC77700.034.34818336
GGCACCC81700.032.6208835
AGGACCG8850.032.25333870
CACCCCC82750.032.1344637
CCCCCGA83900.031.14179219
ATGCCGT5050.030.60455344
ACCCGCG85000.030.60326225
TATCGGA4150.029.9662748
GTTATCG4250.029.261596
TAGTCCG3500.028.8096756
GCAGTCC13100.028.7363685
Produced by FastQC (version 0.11.5)