FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3660329
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN457381.2495598073287948No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC165250.451462149987064No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC138470.3782993277380257No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA119940.32767546305263817No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT72690.19858870609718418No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC47360.129387276389636No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC42700.11665618035974362No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC37160.10152092885639516No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG36930.10089257003946914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT11950.053.28270367
GCGCGGT80400.052.032559
GCCGGTT12100.048.8820273
TACGGCC5450.048.088577
TGGCGCG87800.047.87872715
CGCGGTG88300.047.4934510
CTTAGGC12750.046.25060318
CGGTTCA12900.045.323285
TCTACGG6150.044.8023265
GTAGTCC98850.043.31367527
CTACTCG100300.043.0906437
TGTAGTC99500.042.92759726
TACTCGG100750.042.9077838
TAGTCCC100250.042.57808728
CGTGCCT99700.042.54434220
TTAGGCA14100.042.1262319
ACTCTTC34650.042.0870325
CCTGTAG101400.042.00823224
TCTGGTC34200.041.95479610
GGGGACT35500.041.886791