FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3678108
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN466741.2689676322718093No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC173190.47086708710021563No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC141230.3839745869343695No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA126400.34365494433551164No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT73720.20042913367416074No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC48230.13112719909257695No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC43490.11824013867999525No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG41870.11383570031113822No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC38590.10491807200876102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT11600.056.01058267
GCGCGGT81250.052.38929
GCCGGTT12300.050.0245743
CGGTTCA12400.049.6204645
TGGCGCG86850.048.50725615
CGCGGTG89750.047.5386310
ACTCTTC36200.044.1180655
TGTAGTC98250.044.0485826
GGGGACT35250.043.907861
GTAGTCC98650.043.86452527
TACTCGG100150.043.739638
CTACTCG99850.043.72471237
GGGCGTA2700.043.711221
TCTGGTC35850.043.70350310
TAGTCCC99750.043.49593728
TACGGCC5200.043.321447
TCTACGG5600.043.2290735
CCTGTAG100800.042.71288324
CGTGCCT99850.042.6778120
GCCGAAC15400.042.45170666