FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3775526
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN476521.262128773580158No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC172830.45776403076021727No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC143760.3807681366781741No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA128020.3390785813685298No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT74920.19843592654374517No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC48050.12726703510980986No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC44380.11754653523773906No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG40610.10756117160893608No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC38420.10176065533650146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT12000.053.5551367
GCGCGGT82950.051.885619
TGGCGCG86800.049.6509915
CGGTTCA12150.049.2139245
CGCGGTG90300.047.6623610
GCCGGTT12550.047.1093753
TCTACGG5050.044.5684625
CTTAGGC13650.044.1769418
TGTAGTC100600.044.09163326
CTACTCG102550.043.94165437
GTAGTCC100800.043.93765327
CGTGCCT99450.043.85799820
TAGTCCC101250.043.8514428
TACTCGG103200.043.79967538
TCTGGTC36350.043.7190710
ACTCTTC37350.043.710925
CCTGTAG101450.043.34356724
CACCATA14650.043.1406854
ACTCGGG107300.042.47872539
TACGGCC5300.042.473027