FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3119136
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG69300.2221769105290696No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG36410.11673104346844768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTCG5600.037.13854628
CGCGGCA22150.035.38672632
TGGCCGT11150.034.9736170
TTCGTTG8900.034.2841831
GCGGCAC23000.034.23634733
TCGTTGG8650.034.08070432
AACGGGA6750.033.96268540
CGTTGGA8800.033.50623733
CGTACGT958.481948E-932.81037519
AAACGGG8400.032.24937439
TCGCCGG22150.031.7486748
CGGCACC25350.031.61601434
CCGTCGC22500.031.38271545
CCCGTCG23250.030.50818444
GAAACGG9450.030.1313238
ATGGCCG9800.029.7199269
GGAAACG9300.029.4886337
ATTTCGT7200.029.36978729
CGCGAAC605.305869E-428.8586654
GTTATCG1103.5355697E-828.3339656
Produced by FastQC (version 0.11.5)