FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3123135
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG72060.23072969948465244No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG39430.1262513468037725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT10900.043.78303570
GATTTCG5850.037.32764428
CGCGGCA23950.035.33587332
CGTACGT806.872506E-834.62861619
TTACTCG2450.034.2683657
ATGGCCG10550.033.80829269
GCGGCAC25500.033.3309833
TCGCCGG24500.032.97875648
ATTTCGT6750.032.86992329
TTCGTTG8900.032.7319631
CCGTCGC25050.032.35668645
CGTTGGA8900.032.35267633
CCCGTCG25500.032.0476944
CGGCACC26550.031.88879434
GAAACGG7700.031.11559138
AACGGGA6800.030.65436440
TCGTTGG9600.030.34866532
ACCCGCG28700.030.06194525
AAACGGG7900.029.45872339
CCCCCGA29750.028.86688219
Produced by FastQC (version 0.11.5)