FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3123135
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG83350.26687927355045493No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC71020.22739971214821006No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC56480.1808439276560251No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA45370.1452706975522992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG17700.061.1571124
TCTACGG17950.060.1126755
TACGGCC17800.059.842077
CTACGGC19000.056.608646
ACGGCCA19700.054.5971648
GCGTACC2500.052.7096061
CGGCCAT20850.051.2540669
CGGTATC2900.049.026631
CGGTACT3750.049.010681
GAACGCG22000.048.9268924
TGGCGCG37950.048.20575715
GCCATAC22100.048.03825411
CCTGGTT34250.047.95088270
CGGTATT5450.047.7211571
GCGCGGT38900.047.1196639
CGGTATA4200.047.0621451
CTGAACG23200.046.9838922
CCCGATC23250.046.3476831
TGAACGC23350.046.2411823
GCGTATA3850.045.936261
Produced by FastQC (version 0.11.5)