FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3226621
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG75780.23485869583071578No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG40870.1266650158168561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTCG5850.041.4743228
TGGCCGT10350.039.1273870
CGAAACG901.1459633E-1038.5681437
TTACTCG2400.037.8949257
TTTCGCG852.741217E-936.83504545
CGCGGCA25150.035.99060432
CGTTGGA8200.035.95498733
ATTTCGT6850.035.9306929
TTCGTTG8500.035.89960531
GCGGCAC26000.034.55257833
TCGCCGG25600.033.60854748
TCGTTGG8900.033.5115932
CCGTCGC27050.031.8949645
CGGCACC28400.031.6355934
CCCGTCG27550.031.42866944
ATGGCCG10600.029.9446569
AACGGGA7400.029.5699240
AAACGGG8700.029.13918339
ACCCGCG31700.029.07398225
GAAACGG9000.028.54796838