FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3226621
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG85370.2645801908560069No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC72770.22553005140671928No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC58500.1813042188716927No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA48440.15012609166059476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG18750.061.0658654
TACGGCC18550.060.991587
CTACGGC18900.060.5868536
TCTACGG18950.060.421375
ACGGCCA20750.054.5250058
CGGTATA3350.053.8691331
GCGTATA3850.050.4787641
CGGCCAT22900.049.4058469
GAACGCG23650.047.8546524
GCCATAC23550.047.74762711
GCGCGGT34550.047.1670729
GCGTATT4200.047.0984951
CCTGGTT36050.046.65412570
TGGCGCG35150.046.44885315
CCCGATC24950.045.54445331
CGGTAAT4650.045.525881
ATCTCGT24600.045.5229335
CTGAACG24750.045.4452822
TGAACGC25200.044.90981723
CCATACC25050.044.8884912
Produced by FastQC (version 0.11.5)