FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194061
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG73730.2308346647105362No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG38990.12207030485641947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA24400.034.97320632
GCGGCAC25500.033.7429833
CGGCACC26600.032.08924534
TCGCCGG24750.031.65504548
GATTTCG6650.030.75439328
TCGTTGG8350.030.32679732
CCCGTCG25700.030.3164844
CCGTCGC25700.030.18636145
CGTTGGA8300.029.67919733
GTCGTAC1900.029.15860711
ACCCGCG29850.028.56002825
TTCGTTG9000.028.5165931
TGGCCGT9950.028.34317870
CCCCCGA31450.027.5292619
GCACCCC32250.027.01511636
ATTTCGT7250.026.30166229
AGTCCGC34050.026.032947
AACGGGA6750.025.73665240
AAACGGG8400.025.22476839
CGTTGCG1101.1038574E-625.2187731
Produced by FastQC (version 0.11.5)