FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3156968
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC55890.17703695444489775No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA51170.16208590014216173No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC44950.14238345146355616No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC40840.12936463087367372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT28900.047.933049
TGGCGCG31650.044.6400715
CGGGTAG4050.039.521971
CTACTCG36900.038.69239837
TACTCGG37250.038.2416738
CGCGGTG36650.038.08061210
CGGATAT2500.037.5802351
GTAGTCC38500.037.46883827
TAGTCCC39050.036.7658428
GGGGTAG16850.036.758341
AGGATCG38650.036.58526259
CCGAACT4850.036.40722767
ACTCGGG39900.036.322939
GTACGCA3050.035.69836465
TGTAGTC40700.035.6042926
GATCGCT39800.035.56688761
CGGGTAT3300.034.7965131
CGGTATG2500.034.7965131
CGGTTCA5400.034.6316225
CGTGCCT41200.034.39253220
Produced by FastQC (version 0.11.5)