FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130542
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC55900.17856332865043817No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA49440.15792792430192598No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC45330.14479920729381685No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC40580.1296261158610873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT30750.047.0687759
TGGCGCG32600.043.97283615
CTACTCG36250.040.15439637
TACTCGG36400.039.9972638
GTAGTCC38550.038.05201327
TAGTCCC39100.037.52095828
CGCGGTG39000.037.3782710
GGGGTAG14300.037.2695431
ACTCGGG40100.037.0949339
AGGATCG39050.036.65479759
CGGCATA3050.036.5467531
GGGGACT28850.036.342881
GATCGCT39600.036.1872161
TGTAGTC40950.035.90194326
CGGGGTA2750.035.4669651
CGGCTAT2750.035.4669651
CCTGTAG41900.034.66251424
GGGGTAT9850.034.6568071
GTACGCA2350.034.3665765
CCGAACT5350.034.3434967
Produced by FastQC (version 0.11.5)