FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3138061
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG157930.5032725622605806No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG63170.20130265154182791No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC42350.13495594891240165No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG42280.13473288122824892No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA39870.12705297953099062No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG34860.11108770670805954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA52200.034.17337432
GCGGCAC54300.032.91934633
TCGCCGG52350.032.1461148
CCCGTCG53450.031.7076644
CGGCACC56300.031.69333334
TAGTCCG3650.031.3173356
CCGTCGC54050.031.09963445
CCCCCGA61750.029.51273219
GCACCCC61200.029.33674236
CACCCCC61200.028.94878637
ACCCGCG65050.027.81846425
GTGCCGT5000.027.8170741
CCCCGAC67950.026.82373220
CCGACTA658.4596605E-426.6452834
GTCGTAC4700.026.53147711
GGCACCC68250.026.35260435
GTCGATT4550.025.89430821
AGTCCGC71200.025.784557
AAACCGC8350.025.7194711
GTCGCCT3300.025.62860970
Produced by FastQC (version 0.11.5)