FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3138061
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC55400.17654213860087486No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA50850.1620427391309474No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC44330.14126557769272172No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC40750.12985725898891068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT31000.047.5904669
TGGCGCG32350.045.9256215
CGGGTAG4850.039.5388831
TACTCGG37800.039.2478438
CTACTCG37800.039.05526737
CGCGGTG39150.038.0372210
TAGTCCC40100.037.4459128
GTAGTCC40050.037.40010527
CGGATAT2550.036.9170531
CGGCAAT3400.036.917051
TGTAGTC41450.036.0474926
GGGGTAG14650.035.9370771
CGGGTAT3800.035.7836341
ACTCGGG42400.035.7381739
CGGTAAG2050.035.71651
CGTGCCT42200.035.2200520
TCGCGTA400.002415549535.0133360
CGGTATA1102.5465852E-1134.8661041
AGGATCG42450.034.54687559
GATCGCT42300.034.53894861
Produced by FastQC (version 0.11.5)