FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3137114
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG156330.49832425598814706No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG63620.20279785815880458No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG43510.13869435411017897No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC41250.13149028055722553No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA40350.12862140170870423No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG35930.11453201891929972No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC31450.10025137753999376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA50600.036.56384332
GCGGCAC52450.035.14743433
TCGCCGG51150.033.99926448
CGGCACC55700.033.1056434
CCCGTCG52700.032.8903944
CCGTCGC53250.032.49208545
CCCCCGA61750.030.39756219
GCACCCC61800.030.02139736
CACCCCC61450.029.74911537
TCGCCGT3650.029.6847341
ACCCGCG63050.029.5211325
TAGTCCG4400.029.1129976
GGCACCC67100.027.53729435
TCCGCTA1404.0381565E-1027.46181
CCCCGAC68400.027.34490820
AGTCCGC69000.027.2952887
TCCGCCC72150.026.9193389
CGTCCGT2350.026.7712691
CAGTCCG66400.026.5391856
GTCCGCC72250.026.163318