FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3137114
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC56060.17869927583122577No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA47390.15106240959047074No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC42850.13659050962126337No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC39110.12466872418407493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT29150.046.6791739
TGGCGCG31400.043.22199615
CGGGTAG4850.041.030451
CGCGGTG35750.038.35202410
CTACTCG35300.038.2967137
TACTCGG35550.037.8379438
GGGGTAG15050.037.579561
CGGCATA2250.037.239331
GTAGTCC38400.035.48126227
GGGGTAT11150.035.3815421
AGGATCG37350.035.23795759
TAGTCCC38800.035.1233328
GATCGCT38450.034.63030261
GTCGTAC1700.034.6200453
TGTAGTC39350.034.61968226
ACTCGGG40200.034.50535239
GTACGCA3400.034.0780365
CCTGTAG40800.033.2990524
CGGCAAA6150.032.925021
GGGGACT28900.032.616631
Produced by FastQC (version 0.11.5)