FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4016494
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC98680.24568691002650572No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC83930.2089633396688754No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56900.14166584090502812No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT52490.13068611580149256No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC51470.12814658754625302No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA50510.1257564433060276No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC46980.116967683756032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT10500.049.60677767
GCGCGGT53200.048.624019
TGGCGCG55600.046.89809815
CGGTTCA10850.045.6538435
CGCGGTG60200.043.199710
GCCGGTT11450.042.3644453
CGGCATA2500.040.062021
TACTCGG65250.039.63986638
CTACTCG65350.039.61384637
CGAACTT13900.039.48652668
GTAGTCC66800.038.9893127
TGTAGTC68400.038.42503726
TCTACGG4850.038.2998055
TAGTCCC68550.038.10508728
GTCTACG4900.037.9089934
GGGGTAG18700.037.491191
ACTCGGG70050.037.48881539
CGTGCCT71550.036.62885320
CCGGTTC13550.036.5567634
TGCGACA9250.036.52117524