FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3724629
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG188350.5056879490547918No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG75950.2039129266297395No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG49000.13155672685789646No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC45120.12113958195567934No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA45010.12084425052803917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA53550.035.78472532
GCGGCAC55550.034.56395333
TAGTCCG3350.034.1294256
TCGCCGG53150.034.0891848
CCCGTCG54900.033.34416644
CCGTCGC56050.032.36005445
CGGCACC61050.031.45474234
ACCCGCG66400.029.50503725
CCCCCGA65750.029.46405619
GCACCCC66150.029.30357436
CACCCCC67650.028.30275537
CCCCGAC70150.027.71740220
AGGAGGG96150.026.97581170
CCGCGCG73850.026.62998627
GTCGTAC4700.026.53884111
AGTCCGC75100.025.9272357
CGTCGCC71100.025.71307646
GGCACCC75300.025.55411535
AGTCGTA4900.025.45562410
TCGCCGT3150.025.3888531
Produced by FastQC (version 0.11.5)