FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3708150
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG185950.5014629936760918No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG74130.19991100683629304No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG48940.13197955853997279No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA44790.12078799401318717No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC44710.12057225301026117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA57200.037.93969732
GCGGCAC60300.036.1087933
TCGCCGG57100.035.4368848
CCCGTCG59450.034.28901344
CCGTCGC59500.033.97477745
CGGCACC64300.033.81217234
ACCCGCG70100.031.26280625
CCCCCGA71050.030.92319919
GCACCCC71350.030.62803336
CACCCCC71800.030.53450237
AGGAGGG98000.030.3724670
CCCCGAC75800.029.12412620
CCGCGCG78550.027.90913827
GTACGGC7250.027.71796214
AGTCCGC79650.027.5345237
CGTACGT1900.027.3587919
GGCACCC79650.027.34399435
CGTCGCC74600.027.1977646
CAGTCCG77450.027.0193656
AGGACCG5850.026.6885170
Produced by FastQC (version 0.11.5)