FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3708150
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC47930.12925582837803218No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC46680.12588487520731362No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC43230.11658104445613042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT29300.045.873689
TGGCGCG31650.042.02976615
CTACTCG35700.037.8484837
TACTCGG36050.037.5824638
CGGGTAG5250.037.174361
AGGATCG35650.036.6872259
GGGGTAG20450.035.7882731
CCGAACT5950.034.97393867
CGGCAAT4600.034.850961
ACTCGGG40950.034.61898839
GTAGTCC39500.034.59182427
CGCGGTG39300.034.55357410
GATCGCT38050.034.31538861
CGTGCCT39950.033.91381520
GGGGTAT15950.033.8677021
CGGCATT6550.033.520771
TAGTCCC40900.033.07305528
TGTAGTC41350.033.03972226
GGGGGAT34850.032.1008831
CCTGTAG42600.031.98107724
Produced by FastQC (version 0.11.5)