FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3704994
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG191380.516546045688603No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG75680.20426483821566244No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG49310.13309063388496717No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC46330.1250474359742553No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA45640.1231850847801643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA57100.036.22855432
GCGGCAC60050.034.56814233
CCCGTCG57750.033.98763344
TCGCCGG57400.033.8632548
CCGTCGC59000.032.97486545
CGGCACC63750.032.6220234
ACCCGCG68500.030.42340925
GCACCCC68550.030.14659536
CACCCCC69300.030.07662437
CCCCCGA69450.029.89163619
AGGAGGG97000.028.22875470
GTACGGC6900.028.1231314
CCCCGAC74600.027.9232520
GGCACCC76500.027.00786635
CGTCGCC71800.027.00745246
CCGCGCG77800.026.87981227
TACGGCC7400.026.69118515
AGTCCGC79550.026.1797127
CTACCGG1958.652933E-925.7651370
CGCTTCC9450.025.758031
Produced by FastQC (version 0.11.5)