FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3707781
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG188470.508309417411654No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG75050.20241217051384644No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG49060.1323163369141813No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA45420.12249914436694076No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC43390.11702417160021049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA57750.036.30983432
TCGCCGG57100.034.59470448
GCGGCAC60650.034.57600833
CCGTCGC59700.033.1131845
CCCGTCG59950.033.0804444
CGGCACC63300.033.07589734
CGTACGG1109.695214E-1031.62568943
CCCCCGA69500.030.52287119
GCACCCC69250.030.34904936
CACCCCC70500.030.1611737
ACCCGCG71150.029.92983825
GTTATCG1700.028.537816
CCCCGAC75300.028.49483720
AGGAGGG100000.027.60784170
AACGCGC1404.4019544E-1027.2542526
GGCACCC77450.027.1704135
CCGCGCG78900.027.0397627
CGTACGT1950.026.66339319
AGTCCGC80150.026.4167397
CGTCGCC75200.026.29796846
Produced by FastQC (version 0.11.5)