FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3340508
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG129400.38736623292026245No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC73360.21960731721043628No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG72580.21727234300890763No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG53810.16108328433878918No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC50290.1505459648652241No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT42930.12851338778413343No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT38540.11537167400886333No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG35550.10642093956967023No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG35050.10492416123535703No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA34150.10222996023359322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT15300.041.47891670
CGCGGCA40800.035.34082832
GCGGCAC42000.034.42136833
CCGTCGC41100.033.17790245
CGGCACC43750.032.97170634
TCGCCGG41250.032.84385348
CCCGTCG43700.031.35179744
ATGGCCG15650.031.1600469
CCCCCGA49050.029.7554219
GTTCCGT3050.029.7347431
ACCCGCG49200.029.26572625
GCACCCC50050.029.18229736
TACGGGT11500.029.1650336
CACCCCC50250.029.14350937
AGTCCGC51800.028.4359937
GTACGGG12000.028.2379655
CAGTCCG49950.028.104716
TCGCCGT2750.027.9049131
CCCCGAC51800.027.84440820
GGCACCC53000.027.61681735