FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3340508
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG146190.4376280493865005No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC127020.38024156804893144No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC115310.3451870194593158No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA68910.20628599003504858No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT57910.17335686668015762No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG39260.11752703481027436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG29950.062.6752324
TCTACGG30000.062.5707745
TACGGCC30250.062.0508657
CTACGGC31200.060.0534066
ACGGCCA32650.057.487128
CGGCCAT33950.055.489489
GCCATAC33650.055.162411
GCGCGGT65150.054.0646829
GGGTCTA25950.053.4371262
CCTGGTT52200.051.90455270
CGCGGTG68950.051.18531410
CCATACC36300.051.13462412
GCCGGTT10100.050.9985473
CATACCA36650.050.83492313
GCGTCTA6900.050.6183932
GGCCATA37200.050.36284610
TGGCGCG70600.049.98830815
GAACGCG37500.049.63807324
TGAACGC37750.049.3943323
CTGAACG38350.048.61498322
Produced by FastQC (version 0.11.5)