FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3346979
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG133170.3978811937571165No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC73510.2196308969969635No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG71850.21467120050648658No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG53190.1589194315231736No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC48580.1451458165707045No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT41140.12291681543266329No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT39570.11822601814950137No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG35500.10606579844092241No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG35160.10504995699106567No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA34630.10346643943687725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT15850.048.06720770
CGCGGCA39900.035.96032732
GCGGCAC41500.034.75030533
CGGCACC41900.034.42733834
ATGGCCG17700.033.1424569
TCGCCGG41050.032.5140548
AGGACCG5550.032.24534270
CCGTCGC42750.031.42301845
CCCGTCG43150.031.20580344
CCCCCGA46550.031.19083419
GCACCCC48650.029.80753136
ACCCGCG49300.029.27519825
CTTACCC11100.029.02080770
CACCCCC50400.028.8513437
CCCCGAC50400.028.81509420
AGTCCGC50750.028.8044037
CGCCCTA9200.028.26514661
GTTCCGT3450.028.1568831
CAGTCCG49200.028.026086
CGTTGCG1253.916284E-927.7546421