FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643241
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA126700.34776727644424293No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC76160.20904463910018578No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC52290.1435260527645577No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC51530.14143999806765462No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45560.1250534894617183No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG41230.11316846730699397No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT40880.11220778422289385No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC39720.10902380600130489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT5850.049.13857767
GCGCGGT39300.047.98569
CGCGTAA604.4037733E-947.28267761
TGGCGCG41100.045.29046615
CGGCATA2050.043.781141
TCGCGTA659.03492E-943.5924760
GGGGACT36700.043.0791321
TCTGGTC29550.042.9726210
CGGTTCA6800.041.9972155
GTAGTCC47500.040.24206527
GCCGGTT7200.040.1424673
ACTCTTC32400.040.141925
CGCGGTG47350.039.972910
TACTCGG47550.039.5886138
TGTAGTC48650.039.5625126
CTACTCG47650.039.5591237
GTACGCA8400.039.48736665
TAGTCCC48950.039.0650828
CGGGTAG4700.038.9265171
CGGCAAT3200.038.8347131