FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3592089
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG221240.6159090156173748No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG116470.3242402958278595No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG78370.2181738815491487No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC72140.20083021328257733No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC68300.1901400549930695No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG65040.18106455602853938No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT55240.1537823812271912No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC50640.14097646244288492No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT49770.13855447345541827No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC49490.13777498274680833No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA49080.13663358563777234No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC46460.12933977972149352No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45180.12577639362499093No Hit
GGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAG44140.12288114242158255No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG42230.11756390223070752No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG42210.11750822432294969No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT39090.10882247071272455No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA37110.10331035784469705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG350.001362978139.34122510
AGGACCG9450.038.27659270
CGCGGCA66400.035.76849432
GTTATCG2600.035.747556
GCGGCAC68550.034.61220633
CGGCACC70650.033.4543334
TATCGGA2800.033.1941578
TCGCCGG69700.032.718848
CCCGTCG69950.032.6127244
CCGTCGC69600.032.54487245
GCACCCC75800.031.25461836
CGTACGT3800.030.82065419
TCGCGTG450.004675058630.5978782
TTATCGG3050.030.4733267
GTTCTCG25500.030.37204413
TCGTTTG26200.030.22295617
CACCCCC78550.030.05003737
GGCACCC79250.029.83440235
CCCCCGA81900.029.44155119
CGTTCGT2700.029.3778271
Produced by FastQC (version 0.11.5)