FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3592089
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA126200.3513275979520552No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC72970.20314084645452826No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC52110.14506878866308714No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC48580.13524163794382602No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44900.12499690291638096No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG41510.11555949755142482No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC40000.11135581551570688No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT38470.10709645557223108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT6000.052.6676767
TCGCGTA505.7103534E-849.52115260
GCGCGGT37350.045.795689
GGGGACT35450.042.7959181
TGGCGCG40000.042.76172315
CGGTTCA7500.042.2167975
ACTCTTC32350.041.3841555
GGGGTAG14900.041.2845731
GCCGGTT7450.041.113693
TCTGGTC32200.040.82876610
GTACGCA8450.040.06952365
AGTACGC9150.039.66373464
GTTGATC10450.038.8619410
GGGACTC39100.038.552282
GGTACGA2600.038.3863953
CGCGGTG45150.038.18903710
GGACTCT39050.037.9847223
CGAACTT8700.037.37056768
CTTAGGC8400.037.3025718
TTCTGGT35550.037.0781369