FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4070021
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG184920.45434655005465574No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG84090.2066082705715769No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG69580.1709573488687159No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC54280.13336540523992382No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG49490.12159642419535427No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC48840.11999938083857553No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT44300.10884464723892087No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA43350.10651050694824424No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC43090.10587168960553275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT21800.039.9382570
CGTACGT2400.037.48076219
CGCGGCA58250.036.78711332
GCGGCAC60750.035.3368533
CGGCACC62050.034.60120434
CCCGTCG58650.034.46471444
TCGCCGG59550.033.9471148
CCGTCGC59450.033.89772445
GCACCCC67000.032.3208236
ATGGCCG22100.031.02840669
CACCCCC71200.030.61752537
CCCCCGA71150.030.44007519
ACCCGCG71150.030.42174725
GGCACCC71750.030.22165735
TCGCCGT3700.029.9572641
CGCCCTA13550.028.53286761
CGATCTA3100.028.09231647
CGTTACG500.00758782927.7104681
CCCCGAC78400.027.67199720
AGTCCGC82550.026.775287