FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4014882
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG179090.4460654136285948No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG82630.20580928654939296No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG66980.16682931154639166No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC52260.13016571844452715No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC50650.12615563794901072No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG49730.12386416338014417No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT44300.11033948195737758No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA43500.10834689537575451No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC40640.10122339834645203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT19250.042.3293970
CGCGGCA55700.036.78115532
GCGGCAC57550.035.60680433
TATCGGA1800.034.5817688
CGGCACC59550.034.4212634
CCGTCGC56900.034.0095545
CCCGTCG57450.033.79537244
TCGCCGG57150.033.719148
ATGGCCG19700.032.4007269
GCACCCC65750.031.34742236
CCCCCGA67350.030.76144819
CACCCCC67950.030.59776537
GTTATCG1950.030.1482076
GGCACCC69650.030.03262135
ACCCGCG68650.029.99946225
TTATCGG2150.028.9521777
ACCGTTA1103.5264748E-828.3418641
AGGACCG6250.028.16733770
GTGCCGT6350.027.8211421
CGTACGT2000.027.66989519