FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4014882
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG179180.4462895796190274No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC114300.2846908078493963No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC99280.24727999477942314No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA95820.2386620578139034No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT55080.13718958614474847No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG52960.13190923170344734No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC52240.13011590377998655No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC40210.10015238305882963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG36450.060.5134125
GTCTACG36700.060.1916474
TACGGCC37000.059.707337
CTACGGC38700.057.084536
ACGGCCA40800.054.4005628
CGGCCAT41050.053.5632639
GCCATAC42100.051.6525411
GCGCGGT60600.050.2604529
GGGTCTA31650.049.9164772
CCATACC44600.048.7553912
GCGTCTA8600.048.6394162
CCTGGTT63550.048.45692470
GCCGGTT10400.048.1987883
GGCCATA46500.047.1367310
TGGCGCG64550.047.12954715
CATACCA46700.046.7850413
CGGTTCA11050.045.9921345
GAACGCG47400.045.98953224
CCGAACT10750.045.8673967
ATCTCGT47200.045.8526635