FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3471239
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG175310.5050358099802404No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC121260.3493277184313728No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG112590.3243510458369475No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN88910.256133328762439No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC88420.2547217290425695No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG47600.13712682993017766No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG46880.135052642586696No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG45700.1316532799959899No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT42520.12249228589561248No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA40410.11641376465290924No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT39260.11310082653484824No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG38150.10990312104698063No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG36590.10540904846943698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT18550.045.59104570
TACGCGA405.2596646E-542.76251216
AGGACCG8000.037.19574770
CGCGGCA49950.036.60669332
GCGGCAC51350.035.5632333
TCGCCGG52250.034.5019948
TTACGCG501.9568772E-434.2100115
CGGCACC53450.034.12676634
CCGTCGC52800.033.89526745
GTTCCGT3700.033.403641
CCCGTCG54100.033.23457344
ATGGCCG20400.032.3148869
GTTATCG3100.032.0015266
CCCCCGA58750.031.65536519
ACCCGCG60700.030.55445125
GCACCCC61500.030.54203236
TTATCGG3400.030.1839927
GATAGGC35900.029.87131140
CCCCGAC62250.029.83347320
GGCACCC62300.029.52224535
Produced by FastQC (version 0.11.5)